Coding
REX
Part:BBa_K809401:Design
Designed by: Xiaopeng Xu Group: iGEM12_Shenzhen (2012-09-21)
REX coding sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 768
Illegal AgeI site found at 417 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 133
Design Notes
The signal peptid must be able to work efficiently, and can transport REX into mitochondrial matrix.
Source
SU9 comes from yeast gemome. REX was modified to suitable for expression in yeast.